AbstractThe present study sought to determine the effectiveness of newly developed low calorie chocolate using modified fat through enzymatic interesterification process as cocoa butter substitute of male Sprague Dawley rats (n = 35) compared to cocoa butter as control. Body weight gains did not differ significantly (p>0.05) in all the test groups fed with chocolates which were using different types of fats. Triacylglycerol and cholesterol test showed no significant difference (p>0.05) for all treatment samples. in this study, a pancreatic lipase hydrolysis was performed to determine the fatty acid composition of the sn-2 position of the structure lipids.
Bonanome, A. & Grundy, SM 1988, ‘Effect of dietary stearic acid on plasma cholesterol and lipoprotein levels, N. Engl. J. Med., vol. 318, pp. 1244–1248.
Christensen, MS, Mullertz, A & Hoy C 1995, ‘Absorption of triglycerides with defined or random structure by rats with biliary and pancreatic diversion’, Lipids, vol. 30, no. 6, pp. 521–526.
Decker, EA 1996, ‘The role of stereospecific saturated fatty acid positions on lipid metabolism’, Nutr. Rev., vol. 54, pp. 108–110.
Hegsted, DM, Ausman, LM, Johnson, JA & Dallal, GE 1993, ‘Dietary fat and serum lipids: an evaluation of the experimental data’, Am. J. Clin. Nutr., vol. 57, pp. 875–883.
Hunter, JE 2001, ‘Studies on effects of dietary fatty acids as related to their position on triglycerides’, J. Lipid, vol. 36, pp. 655–668.
Jandacek, RJ, Whiteside, JA, Holcombe, BN, Volpenhein, RA & Taulbee, JD 1987, ‘The rapid hydrolysis and efficient absorption of triglycerides with octanoic acid in the 1- and 3-positions and long-chain fatty acid in the 2-position’, Am. J. Clin. Nutr., vol. 45, pp. 940–945.
Katan, MB., zock, PL & Mensink, RP 1994 ‘Effects of Fats and fatty acids on blood lipids in humans: an overview’, Am. J. Clin. Nutr., vol. 60 (suppl.), pp. 1017S–1022S.
Mattson, FH & Grundy, SM 1985, ‘Comparison of effects of dietary saturated, monounsaturated, and polyunsaturated fatty acids on plasma lipids and lipoproteins in man’, J. Lipid Res., vol. 26, pp. 194–202.
Mu, H & Hoy, C-E 2002, ‘Distribution of medium-chain FA in different lipid classes after administration of specific structured TAG in rats,’ Lipids, vol. 37, pp. 329–331.
Osborn HT & Akoh CC 2002, ‘Structured lipids– novel fats with medical, nutraceutical, and food applications’, in Comprehensive reviews in food science & food safety, vol. 3.
Summers, LKM, Fielding, BA, Herd, SL, ilie, V, Clark, ML, Quinlan, PT & Frayn, KN 1999, ‘Use of structured triacylglycerols containing predominantly stearic and oleic acids to probe early events in metabolic processing of dietary fat’, J. Lipid Res., vol. 40, pp. 1890–1898.
World Health Organization 2009, ‘Fact sheet # 317, cardiovascular diseases updated Sept 2009, viewed May 2010, <http://www.who.int/mediacentre/factsheets/fs317/en/index.html>.