Biodegradation of NR Latex-based Materials via a Carbon Dioxide Evolution Method


Natural rubber latex
CO2 evolution
aerobic system


NR as a natural polymer has biodegradable characteristics and their existence was examined using CO2 evolution methods. The CO2 molecule produced by micro-organism metabolisms in the degradation system was quantified using a conventional acidimetric method. An aerobic system was determined as most suitable condition to be examined under this method. The presence of O2 in the system would help micro-organisms to destabilize the natural polymer. The material of LATZ, HA film and NR gloves showed significant weight loss and were able to produce CO2 evolution curves after 45 days in the biodegradation system compared to synthetic polyisoprene films. Gel permeation chromatography, fourier transform infrared spectroscopy and scanning electron micrograph were used to characterize the degraded sample at molecular and physical levels.


Barnard, D & Lewis, PM 1988, ‘Oxidative ageing’, Natural Rubber Science and Technology, pp. 621–678.

Ellis, ER & Schurin, B 1969, Appl. Optics, vol. 51, pp. 109.

Flemming, H-C 1997, ‘Relevance of biofilm for the biodeterioration of surface of polymeric materials’, Polymer Degradation and Stability, vol. 59, pp. 309–315.

Freijer, JI & Bouten W 1991, ‘A comparision of field methods for measuring soil carbon dioxide evolution: experimental and simulation’, Plant and Soil, vol. 135, pp. 133–142.

Imal, P, Hoffmann, J & Druzˇbik, M 2007, ‘Evaluating the aerobic biodegradability of plastics in soil environments through GC and IR analysis of gaseous phase’, Polymer Testing, vol. 26, pp. 729–741.

International Standard ISO 1999, 14855:1999 (E) Determination of the ultimate aerobic biodegradability and disintegration of plastic materials under controlled composting conditions—Method by analysis of evolved carbon dioxide.

Jendrossek, D, Tomasi, G & Kroppenstedt, RM 1997, ‘Bacterial degradation of natural rubber: a privilege of actinomycetes’, FEMS MicrobiolLett, vol. 150, pp. 179–188.

Kamiński, M, Kartanowicz, R, Jastrzębski, D & Kamiński, MM 2003, ‘Determination of carbon monoxide, methane and carbon dioxide in refinery hydrogen gases and air by gas chromatography’, Journal of Chromatography A, vol. 989, pp. 277–283.

Koltoff, IM 1936, Textbook of quantitative inorganic analysis. MacMillan, New York, NY.

Malaysian Rubber Board 2008, Malaysian Rubber Statistics 2008.

Massardier-Nageotte, V, Pestre, C, Cruard-Pradet T & Bayard, R 2006, ‘Aerobic and aerobic biodegradability of polymer film and physicchemical characterization’, Polymer Degradation and Stability, vol. 91, pp. 620–627.

Mohr, SA, Zottola, EA & Reieneccius, GA 1993, ‘The use of gas chromatography to measure carbon dioxide production by dairy starter culture’, Journal of Dairy Science, vol. 11, pp. 3350–3353.

Pagga, U 1997a, ‘Biodegradability and composability of polymeric materials in the context of the European packaging regulation’, Polymer Degradation and Stability, vol. 59, pp. 371–376.

Pagga 1997b, ‘Testing biodegradability with standardized methods’, Chemosphere, vol. 35, no. 12, pp. 2953–2972.

Satienperakul, S, Cardwell, TJ, Cattrall, RW, McKelvie, ID, Taylor, DM & Kolev, SD 2004, Determination of carbon dioxide in gaseous sample by gas diffusion-flow injection’, Talanta, vol. 62, pp. 631–636.

Shabinah FMS & Hashim, MYA 2010, ‘Biodegradation rate of NR latex film via carbon dioxide evolution method’, in Malaysian Science and Technology Congress 2010.

Singh, B & Sharma, N 2008, ‘Mechanistic implications of plastic degradation’, Polymer Degradation and Stability, vol. 93, pp. 561–584.

Tsuchii, A & Tokiwa, Y 1999, ‘Colonization and disintegration of tire rubber by a colonial mutant of Nocardia’, J. Biosci. Bioeng., vol. 87, pp. 542–544.

Umbreit, WW 1946, Manometric and biochemical techniques, Burgess Publ. Co., Minneapolis, MN.


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